Resources

EcoFAB 2.0

Novak, V., Andeer, P. F., Bowen, B. P., Ding, Y., Zhalnina, K., Tomaka, C., Golini, A. N., Kosina, S. M., & Northen, T. R. (2024). Reproducible growth of Brachypodium distachyon in fabricated ecosystems (EcoFAB 2.0) reveals that nitrogen form and starvation modulate root exudation. Science Advances. 10, eadg7888. DOI:10.1126/sciadv.adg7888

Andeer, P.F., & Novak, V. (2023). Assembly and sterilization of EcoFAB 2.0 for plant growth experiments. protocols.io. https://www.protocols.io/view/assembly-and-sterilization-of-ecofab-2-0-for-plant-q26g7p693gwz/v1

EcoFAB 1.0

Jabusch, L.K., Kim, P.W., Chiniquy, D., Zhao, Z., Wang, B., Bowen, B., Kang, A.J., Yoshikuni, Y., Deutschbauer, A.M., Singh, A.K., et al. Microfabrication of a Chamber for High-Resolution, In Situ Imaging of the Whole Root for Plant–Microbe Interactions (2021). International Journal of Molecular Sciences., 22(15), 7880. https://doi.org/10.3390/ijms22157880

Gao, J., Sasse, J., Lewald, K. M., Zhalnina, K., Cornmesser, L. T., Duncombe, T. A., … Northen, T. R. (2018). Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions. Journal of Visualized Experiments, (134), 1–16. https://doi.org/10.3791/57170

Marx, V. (2019). Engineers embrace microbiome messiness. Nature Methods. https://doi.org/10.1038/s41592-019-0460-5

Sasse, J., Kant, J., Cole, B. J., Klein, A. P., Arsova, B., Schlaepfer, P., … Northen, T. R. (2019). Multilab EcoFAB study shows highly reproducible physiology and depletion of soil metabolites by a model grass. New Phytologist, 222(2), 1149–1160. https://doi.org/10.1111/nph.15662

Zengler, K., Hofmockel, K., Baliga, N. S., Behie, S. W., Bernstein, H. C., Brown, J. B., … Northen, T. R. (2019). EcoFABs : advancing microbiome science through standardized fabricated ecosystems. Nature Methods. https://doi.org/10.1038/s41592-019-0465-0

Zhalnina, K., Zengler, K., Newman, D., & Northen, T. R. (2018). Need for Laboratory Ecosystems To Unravel the Structures. MBio, 9(4), 1–8. https://doi.org/10.1128/mBio.01175-18

Model Microbes

Coker, J., Zhalnina, K., Marotz, C., Thiruppathy, D., Tjuanta, M., D’Elia, G., Hailu, R., Mahosky, T., Rowan, M., Northen, T. R., & Zengler, K. (2022). A reproducible and tunable synthetic soil microbial community provides new insights into Microbial Ecology. mSystems, 7(6). https://doi.org/10.1128/msystems.00951-22