EcoFAB 2.0
Lin, H. H., Torres, M., Adams, C. A., Andeer, P. F., Owens, T. K., Zhalnina, K., Jabusch, L.K., Carlson, H. K., Kuehl, J. V., Deutschbauer, A. M., Northen, T. R., Glass, N. L., Mortimer, J. C. (2024) Impact of Inoculation Practices on Microbiota Assembly and Community Stability in a Fabricated Ecosystem. APS Publications. https://doi.org/10.1094/PBIOMES-06-23-0050-R
Andeer, P.F., & Novak, V. (2023). Assembly and sterilization of EcoFAB 2.0 for plant growth experiments. protocols.io. https://www.protocols.io/view/assembly-and-sterilization-of-ecofab-2-0-for-plant-q26g7p693gwz/v1
Novak, V., Andeer, P. F., Bowen, B. P., Ding, Y., Zhalnina, K., Tomaka, C., Golini, A. N., Kosina, S. M., & Northen, T. R. (2023). Reproducible growth of Brachypodium distachyon in fabricated ecosystems (EcoFAB 2.0) reveals that nitrogen form and starvation modulate root exudation. bioRxiv. https://doi.org/10.1101/2023.01.18.524647
EcoFAB 1.0
Gao, J., Sasse, J., Lewald, K. M., Zhalnina, K., Cornmesser, L. T., Duncombe, T. A., … Northen, T. R. (2018). Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions. Journal of Visualized Experiments, (134), 1–16. https://doi.org/10.3791/57170
Marx, V. (2019). Engineers embrace microbiome messiness. Nature Methods. https://doi.org/10.1038/s41592-019-0460-5
Sasse, J., Kant, J., Cole, B. J., Klein, A. P., Arsova, B., Schlaepfer, P., … Northen, T. R. (2019). Multilab EcoFAB study shows highly reproducible physiology and depletion of soil metabolites by a model grass. New Phytologist, 222(2), 1149–1160. https://doi.org/10.1111/nph.15662
Zengler, K., Hofmockel, K., Baliga, N. S., Behie, S. W., Bernstein, H. C., Brown, J. B., … Northen, T. R. (2019). EcoFABs : advancing microbiome science through standardized fabricated ecosystems. Nature Methods. https://doi.org/10.1038/s41592-019-0465-0
Zhalnina, K., Zengler, K., Newman, D., & Northen, T. R. (2018). Need for Laboratory Ecosystems To Unravel the Structures. MBio, 9(4), 1–8. https://doi.org/10.1128/mBio.01175-18
Model Microbes
Coker, J., Zhalnina, K., Marotz, C., Thiruppathy, D., Tjuanta, M., D’Elia, G., Hailu, R., Mahosky, T., Rowan, M., Northen, T. R., & Zengler, K. (2022). A reproducible and tunable synthetic soil microbial community provides new insights into Microbial Ecology. mSystems, 7(6). https://doi.org/10.1128/msystems.00951-22